Table 1 List of primers used and respective forward and reverse sequences

From: The osteogenesis of Ginsenoside Rb1 incorporated silk/micro-nano hydroxyapatite/sodium alginate composite scaffolds for calvarial defect

Gene

Forward sequence

Reverse sequence

β-actin

5′-GTAAAGACCTCTATGCCAACA-3′

5′-GGACTCATCGTACTCCTGCT-3′

Runx2

5′-ATCCAGCCACCTTCACTTACACC-3′

5′-GGGACCATTGGGAACTGATAGG-3′

ALP

5′- TTTGCTACCTGCCTCACTTCCG-3′

5′-GGCTGTGACTATGGGACCCAG-3′

COL I

5′-CTGCCCAGAAGAATATGTATCACC-3′

5′-GAAGCAAAGTTTCCTCCAAGACC-3′

OCN

5′-GCCCTGACTGCATTCTGCCTCT-3′

5′-TCACCACCTTACTGCCCTCCTG-3′

OPN

5′-CCAAGCGTGGAAACACACAGCC-3′

5′-GGCTTTGGAACTCGCCTGACTG-3′

VEGF

5′-GGCTCTGAAACCATGAACTTTCT-3′

5′-GCAATAGCTGCGCTGGTAGAC-3′

ANG1

5′-GGACAGCAGGCAAACAGAGCAGC-3′

5′-CCACAGGCATCAAACCACCAACC-3′

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