Fig. 3
From: Four-Octyl itaconate ameliorates periodontal destruction via Nrf2-dependent antioxidant system
4-OI scavenges excess ROS and mitigates oxidative damage in vitro. Fluorescent images (a) and semi-quantitative analysis (b) of the intracellular ROS in RAW 264.7 measured by DCFH-DA staining. (Scale bar = 400 µm). c Flow cytometry images of intracellular ROS in RAW264.7 stained by DCFH-DA. d Representative fluorescent images and 3D surface plot images of 8-OHdG and nuclei in RAW 264.7. (Nucleus: blue, 8-OHdG: red. Scale bar = 400 µm). e The MDA content of RAW 264.7. f Schematic diagram of applying 4-OI to reduce LPS-induced oxidative damage in vitro. Data are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.000 1
