Fig. 5
From: Four-Octyl itaconate ameliorates periodontal destruction via Nrf2-dependent antioxidant system
The role of Nrf2 exerted in the protection of 4-OI against periodontal inflammation. a The Nrf2 protein expression in nuclei fraction lysates quantified by western blotting. b qRT-PCR analysis of NQO1, HO-1, Prdx and GCLM in RAW264.7. c The expression of Nrf2 downstream ARE-dependent proteins in RAW264.7 measured by western blotting. d Transfection effects of siRNA or negative control siRNA (NC) analyzed by western blotting. Nrf2 expression in nuclei fraction lysates (e) and downstream proteins in cytosol fraction lysates (f) after siRNA transfection as detected by western blotting. g Representative images of histological analysis in periodontal tissue. (Scale bar = 100 or 25 µm). h Schematic diagram of the nuclear translocation of Nrf2. Data are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.000 1. sMaf small musculoaponeurotic fibrosarcoma
