Fig. 10 | International Journal of Oral Science

Fig. 10

From: GPCR/endocytosis/ERK signaling/S2R is involved in the regulation of the internalization, mitochondria-targeting and -activating properties of human salivary histatin 1

Fig. 10

Knockdown (KD) of TMEM97 alter the mitochondrial-targeting and -activating properties of Hst1. HO1N1 cells were transfected with siRNA to downregulate the expression of TMEM97, and subsequently cultured with in medium supplemented with F-Hst1. a Typical image of subcellular localization of F-Hst1 with control siRNA transfection and loss of both TMEM97 in HO1N1 cells. Bar = 10 μm. F-Hst1 (in red); mitochondrial (in green). b Mean intracellular fluorescence intensity of F-Hst1 was quantitated using Fiji software. c Mander’s overlap coefficients of co-localization F-Hst1 with mitochondria in the TMEM97 KD. d Effects of TMEM97 on Hst1-stimulated cellular metabolic activity quantified by PrestoBlue assay. Data are presented as mean ± SD; ****P < 0.000 1

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