Fig. 3 | International Journal of Oral Science

Fig. 3

From: GPCR/endocytosis/ERK signaling/S2R is involved in the regulation of the internalization, mitochondria-targeting and -activating properties of human salivary histatin 1

Fig. 3

Trafficking and intracellular distribution of F-Hst1. Before adding F-Hst1 to the cell culture medium, nuclear DNA was stained with NucBlue™ live cell reagent. The cell membrane was labeled using PKH67 Green Fluorescent Cell Linker Kit. Representative CLSM images showed that an endocytic vesicle was formed from the beginning to the later time points throughout the very fast translocation process (as indicated in the yellow arrow). After uptake, the loaded vesicle pinched off from the cell membrane and was released into the cytoplasm. Bar = 2 μm. F-Hst1 (in red); nuclei (in blue); cell membrane (in green)

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