Fig. 1

circ_0003204 negatively regulated hASC osteogenesis. a Images of ALP and ARS stainings after transfection (scale bar = 200 μm). b–d Western blot after 7-day osteogenic induction of hASCs detected the protein expressions and the results were quantitated. e, f The expressions of ALPL and RUNX2 after 7-day osteogenic induction of hASCs were evaluated by RT-qPCR. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.000 1)