Fig. 3 | International Journal of Oral Science

Fig. 3

From: circ_0003204 regulates the osteogenic differentiation of human adipose-derived stem cells via miR-370-3p/HDAC4 axis

Fig. 3

circ_0003204 inhibited hASC osteogenesis through sponging miR-370-3p. a Bioinformatic analysis predicted the target relationship. b RT-qPCR evaluated the expression of miR-370-3p. c The putative binding sequences. d Luciferase reporter genes were co-transfected with miR‐370‐3p mimic/mimic-NC and mut-type/wild-type circ_0003204 into cells, and the luciferase activities were measured. e RT-PCR evaluated the expression of circ_0003204 and miR-370-3p. f Images of ALP and ARS stainings after transfection. g, h ALP and ARS activity analyses were measured. i RT-qPCR evaluated the mRNA expressions after 7-day osteogenic induction of hASCs (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.000 1)

Back to article page