Fig. 4 | International Journal of Oral Science

Fig. 4

From: circ_0003204 regulates the osteogenic differentiation of human adipose-derived stem cells via miR-370-3p/HDAC4 axis

Fig. 4

miR-370-3p regulated hASC osteogenesis by targeting HDAC4. a Bioinformatic analysis predicted the target relationship. b The expressions of HDAC4 were evaluated by RT-qPCR. c circ_0003204 siRNAs carried Cy3 reporter (red) and transfection efficiency was confirmed by fluorescence microscopy (scale bar = 100 μm). d Images of ALP and ARS stainings after transfection. e, f ALP and ARS activity analyses were detected. g RT-qPCR evaluated the mRNA expression levels of BGLAP after 7-day osteogenic induction of hASCs. h The expression of HDAC4 was regulated by circ_0003204. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.000 1)

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