Fig. 5 | International Journal of Oral Science

Fig. 5

From: circ_0003204 regulates the osteogenic differentiation of human adipose-derived stem cells via miR-370-3p/HDAC4 axis

Fig. 5

HDAC4 inhibited hASC osteogenesis. a, b Images of ALP and ARS stainings after transfection (scale bar = 200 μm). c Western blot evaluated the protein expressions after 7-day osteogenic induction of hASCs and histograms show quantification of the band intensities. d RT-qPCR evaluated the mRNA expressions after 7-day osteogenic induction of hASCs. e, f Immunofluorescence (IF) staining of COL1A1 (green) and RUNX2 (red) was performed after 7-day osteogenic differentiation (scale bar = 40 μm). The 4’,6-diamidino-2-phenylindole (DAPI) stained nuclei (blue) (*P < 0.05, **P < 0.01, ****P < 0.000 1)

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