Fig. 7

circ_0003204 regulated hASC osteogenic differentiation in vivo. a Three-dimensional (3D) reconstructed images of the defect sites of nude mice in each group. b The bone mineral density (BMD) was measured on the regions of interest (ROI) of the entire defect area. c The bone volume/total volume (BV/TV) ratio was calculated on the ROI of the entire defect area. d The trabecular spacing (Tb.Sp) was measured on the ROI of the entire defect area. e Representative images of hematoxylin and eosin (HE) staining in the defect area. f Representative images of Masson’s trichrome staining in the defect area, where collagen and new bone were stained blue, and the muscle and cytoplasm were stained red. g Histochemistry staining of COL1A1 in the defect area (*P < 0.05, **P < 0.01, ****P < 0.000 1)