Fig. 1 | International Journal of Oral Science

Fig. 1

From: Bone morphogenetic protein 7 mediates stem cells migration and angiogenesis: therapeutic potential for endogenous pulp regeneration

Fig. 1

Identification of the stem cell sources. a The schematic diagram of the stem cells residing in the dental pulp and root apex. b Double-immunofluorescent labeling CD90 (MSCs) and NG2 (pericytes) in the apical tissue. Both CD90-positive and NG2-positive regions were highlighted by white arrows and enlarged in the white rectangle frame. c h-DPSCs isolated from dental pulp tissue. d The evaluation of h-DPSCs proliferation by CCK8 assay under 10% FBS/α-MEM culture. e The colony formation assay to evaluate the self-renewal of h-DPSCs. f The flow cytometry to detect MSCs markers (CD90 and CD73) expression. g The in vitro osteogenic, adipogenic, and neural differentiation potential of h-DPSCs evaluated by alizarin red staining, oil red staining, and immunofluorescent labeling tubulin βIII respectively. Scale bar: b 200 μm, c 100 μm, g 400 μm (alizarin red), and 50 μm (oil red and tubulin βIII labeled)

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