Fig. 2

miR-330-5p inhibited pro-inflammatory polarization and promoted anti-inflammatory polarization of microglia. a–c The level of IL-6, TNF-α and IL-10 secreted by microglia. d Real-time PCR assay of M1 polarization marker CD86 mRNAs in microglia. e Real-time PCR assay of M1 polarization marker MHCII mRNAs in microglia. f Real-time PCR assay of M2 polarization marker IL-10 mRNAs in microglia. g, h Immunofluorescent staining for CD86 and CD206. Scale bar: 25 μm