Fig. 2

Effects of IFN-γ on migration and invasion of SACC-83. a Representative images showing the morphological changes of SACC-83 upon IFN-γ treatment for 48 h. b Wound healing assay of SACC-83 upon IFN-γ treatment for 24 h. Control group (C) and IFN-γ-treated group (IFN-γ) were quantified by Image J. Wound size was presented as the wound healing rate (n = 7). c The migration and invasion of SACC-83 upon IFN-γ treatment for 18 h were determined by transwell assay. The numbers of invading cells of IFN-γ-treated group (IFN-γ) were normalized to the control group (C) (n = 5). d Western blot analysis of EMT-related proteins. ***, P < 0.001; ****, P < 0.000 1; scale bar: 100 μm