Fig. 4 | International Journal of Oral Science

Fig. 4

From: Interferon-γ induces immunosuppression in salivary adenoid cystic carcinoma by regulating programmed death ligand 1 secretion

Fig. 4

Effects of IFN-γ-induced sEVs derived from SACC-83 on T cells. a Immunofluorescence staining of CellMask-labeled Jurkat T cells (stimulated with PMA + Ion) after incubation with CFSE-labeled sEVs derived from SACC-83 for 12 h. Scale bar: 10 μm. b Representative histogram of Jurkat T cells with PMA + Ion stimulation after incubating with CFSE-labeled control sEVs and IFN-γ-induced sEVs for 12 h. c Comparison of the proportion of sEVs-bound cells (CFSE positive) between control sEVs (C) and IFN-γ-induced sEVs (IFN-γ) (n = 3). d CCK8 assay showing the relative proliferation of Jurkat T cells with PMA + Ion stimulation after incubation with control sEVs and IFN-γ-induced sEVs for 24 h (n = 4). e Representative histogram of Jurkat T cells with PMA + Ion stimulation after incubating with IFN-γ-induced sEVs for 12 h. f The mean fluorescence intensity (MFI) of Annexin V of Jurkat T cells after incubation with IFN-γ-induced sEVs (n = 3). *, P < 0.05; ****, P < 0.000 1

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