Fig. 2

MSCs-ApoEVs promote muscle regeneration of TA injury. a The schematic graph of establishment and treatment for TA injury model. b The representative images of the TA sample by H&E staining after 3 days’ CTX injection. c The representative fluorescence images of the TA sample after MSCs-ApoEVs treatment, MSCs-ApoEVs were pre-stained by PKH26, the scale bar indicates 100 μm. d, e The representative images of the TA sample by H&E staining after PBS or MSCs-ApoEVs treatment on Day 7 or Day 14, yellow arrows indicated multinucleated cells. f, g Analysis of myofiber CSA and multinuclear cells proportion of different treated groups in Day 7 or Day 14 h, i The representative images and analysis of the TA sample by Masson staining after PBS or MSCs-ApoEVs treatment on Day 7 or Day 14. j, k Analysis of collagen volume fraction of different treated groups on Day 7 or Day 14. n = 3 per group; the scale bar indicated 100 μm in the low magnification field and 50 μm in the high magnification field. Data were shown as mean ± SD; ns not significant; *P < 0.05, **P < 0.01, ***P < 0.001. TA tibialis anterior, CTX cardiotoxin, H&E hematoxylin & eosin, LPF low power field, HPF high power field, CSA cross-sectional area