Fig. 5

Inhibition of apoptosis in C2C12 myoblasts impaired the fusion process. a, b Z-VAD inhibited the apoptosis ratio of C2C12 myoblasts in the fusion medium. The relative PE-Annexin V expression of C2C12 myoblasts in the growth medium, fusion medium, and fusion medium with Z-VAD was examined by flow cytometry (a). The analysis of apoptosis ratio of C2C12 myoblasts in different treated groups (b). c, d Z-AVD impaired the fusion ratio of C2C12 myoblasts in the fusion medium. The representative immunofluorescence images of myosin staining of C2C12 myoblasts in control and Z-VAD treated groups (c). The analysis of the fusion index of C2C12 myoblasts in different treated groups (d). e, f Myo-ApoEVs rescue Z-VAD impaired fusion process of C2C12 myoblasts. The representative immunofluorescence images of myosin staining of C2C12 myoblasts in control and Z-VAD treated groups with or without Myo-ApoEVs (e). The analysis of fusion index of C2C12 myoblasts in different treated groups (f). n = 3 per group; data were shown as mean ± SD; ns not significant; the scale bar indicates 20 μm; *P < 0.05, **P < 0.01, ***P < 0.001