Fig. 1
The 810 nm low-intensity NIR light promotes bone regeneration. a, b Representative images (a) of micro-CT reconstruction of skull in rats with or without 810 nm NIR light irradiation at 7, 14, 28 days and analysis (b) of bone volume/total volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp) of skull defect area. c, d Representative ALP staining images (c) and quantitative detection of ALP activity (d) in BMSCs with or without 810 nm NIR light irradiation for 7 days. e At 3 weeks after the induction of osteogenic differentiation in BMSCs with or without 810 nm NIR light irradiation, each wells was stained with ARS (left). The ARS-positive areas were quantified from each individual culture plate (right). f, g Representative ALP staining images (f) and quantitative detection of ALP activity (g) in MC3T3-E1 cells with or without 810 nm NIR light irradiation for 7 days. h At 3 weeks after the induction of osteogenic differentiation in MC3T3-E1 cells with or without 810 nm NIR light irradiation, each wells was stained with ARS (left). The AR-positive areas were quantified from each individual culture plate (right). Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bar: 1 mm for (a) and 100 μm for (c, e, f, h)
