Fig. 7 | International Journal of Oral Science

Fig. 7

From: Porphyromonas gingivalis bacteremia increases the permeability of the blood-brain barrier via the Mfsd2a/Caveolin-1 mediated transcytosis pathway

Fig. 7

Mfsd2a negatively regulated the number of Porphyromonas gingivalis (P. gingivalis) internalized, cell permeability, and Cav-1 protein expression in brain microvascular endothelial cells (BMECs). a Mfsd2a knockdown significantly promoted P. gingivalis internalization into BMECs. I and II: Flow cytometry and quantification of fluorescein isothiocyanate (FITC)-positive cells. III: Quantity of P. gingivalis detected by RT-qPCR. b Mfsd2a overexpression significantly inhibited P. gingivalis internalization into BMECs. I and II: FITC-positive cells. III: Quantity of P. gingivalis detected by RT-qPCR. c Western blot (I) and quantification (II) showed that Cav-1 protein expression decreased after overexpressing Mfsd2a in BMECs infected with P. gingivalis for 24 h. Values are expressed as the mean ± standard deviation. The results represent three independent experiments. *P < 0.05, **P < 0.01,***P < 0.001 when compared to the control group. si, Mfsd2a knockdown with siRNA. pc, transfected with Mfsd2a plasmids

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