Fig. 2

Effect of fibroblast growth factor 10 (FGF10) treatment on DEOs. a–c DEOs were grown in +NJ or FGF10-supplemented medium (+NFJ) for 2 weeks. Bright-field images of DEOs (a, top, scale bar = 50 μm). H&E staining of paraffin sections of DEOs (a, bottom, scale bar = 50 μm). Number of buds per DEOs (b, n = 50). Confocal image of paraffin sections of DEOs costained for Sox2 (c red), cytokeratin 14 (Krt14, c green), and TO-PRO-3 (c blue). Scale bar = 100 μm (c). Volcano plots of differentially expressed genes (DEGs) of DEOs grown in +NFJ compared with those of DEOs grown in +NJ. Fold changes are shown for all DEGs (d) or for genes related to specific ontology (GO) terms (e–i) as red (|fold change| > 2 and P-value < 0.001), blue (|fold change| < 2 or P-value > 0.001), and gray (not related to the indicated GO term) dots. Vertical dashed lines indicate −2-fold and +2-fold changes. A horizontal dashed line indicates a P-value of 0.001