Fig. 3

DEOs cultured in Jagged1-depleted, FGF10-supplemented medium form robust basal epithelial layers. a–g DEOs were grown in Jagged1-depleted, FGF10-supplemented medium (+NF) for 2 weeks. Bright-field image of a DEO (a, scale bar = 100 μm). H&E staining of paraffin sections of a DEO (b, scale bar = 100 μm). Maximum intensity projection of serial Z-section confocal images of a whole-mount DEO stained for Ki67 (c red), Krt14 (c green), and DAPI (c blue, scale bar = 50 μm). Confocal image of paraffin sections of a DEO costained for Ki67 (d, red) and Krt14 (d, green, scale bar = 50 μm). Confocal image of paraffin sections of a DEO costained for P-cadherin (P-cad, e red), E-cadherin (E-cad, e, green), and TO-PRO-3 (e, blue, scale bar = 50 μm). Higher magnification (f scale bar = 25 μm). Confocal image of paraffin section of a DEO costained for P63 (g red), Krt10 (g green), and TO-PRO-3 (g blue, scale bar = 25 μm)