Fig. 3

PRX1 deficiency in vivo induced retarded proliferation in M3. a Representative images of EdU labelled proliferating cells (green) in tooth germs at different developmental stages. Samples were collected 2 h after EdU administration (n = 3 per developmental stages). Scale bar: 100 μm. b Quantification of EdU-positive cells in dental mesenchyme and epithelium between M1 and M3 at bud, cap and bell stages (n = 3 per developmental stages). The error bar represents the standard deviation of the mean. ns no statistical significance, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.000 1. c Pcna mRNA expression between M1 and M3 was verified by RT-qPCR. M3 showed significantly lower Pcna expression in early bell stage. ns no statistical significance, ****P < 0.000 1. d Representative images and quantification of TUNEL labelled apoptotic cells (green) in tooth germs of M1 and M3 at cap stage (n = 3 per developmental stages). Scale bar: 100 μm. ns no statistical significance. e Representative images and quantification of TUNEL labelled apoptotic cells (green) in tooth germs of M1 and M3 at early bell stage (n = 3 per developmental stages). Scale bar: 100 μm. ns no statistical significance