Fig. 6

PRX1-positive stem cells induced differential Wnt5a expression between M1 and M3. a Fluorescence in situ hybridization of Wnt5a (red) in M1 tooth germ at E14.5 and E17.5 (n = 3 per developmental stages). Wnt5a was specifically expressed in the developing molar mesenchyme. epi epithelium, mes mesenchyme. Scale bar: 100 μm. b Wnt5a mRNA expression between M1 and M3 was evaluated by RT-qPCR (n = 3 per developmental stages). M3 showed significantly lower Wnt5a expression than control from early bell stage to matrix secretion stage. The error bar represents the standard deviation of the mean. *P < 0.05. c Wnt5a and β-catenin mRNA expression levels in cultured M1 cells were evaluated by RT-qPCR after Prx1 knockdown (n = 3). **P < 0.01, ***P < 0.001, ****P < 0.000 1