Fig. 4
CC ameloblastoma cells is endowed with stemness in ameloblastoma. a UMAP plot showing the expression of selected genes related to the cell cycle in CC AM cells. b–d Violin plot of stemness-related gene signature scores in the five epithelial clusters, *P < 2.22e-16 by pairwise Wilcoxon rank-sum tests. e DiDlow and DiDhigh AM cells sorted by using DiD staining. f Representative images and quantification of colony formation in sorted DiDlow and DiDhigh AM cells, *P < 0.05 by Student’s t test. g The CCK8 assay showing the proliferative activity of sorted DiDlow and DiDhigh AM cells, ***P < 0.001 by two-way ANOVA. h Representative images showing spheres from sorted DiDlow and DiDhigh AM cells; scale bar, 100 μm. i Quantification of sphere numbers from sorted DiDlow and DiDhigh AM cells, ***P < 0.001 by Student’s t test. j Quantification of sphere diameter from sorted DiDlow and DiDhigh AM cells, ***P < 0.001 by Student’s t test
