Fig. 6

Targeting EZH2 inhibits proliferation and stemness in ameloblastoma cells. a Heatmap of the expression of stem cell-associated transcription factors in tumor cells. b Heatmap of the differentiated regulons of each epithelial cluster inferred by SCENIC. c Representative images of colony formation of sorted DiD^low AM cells treated with EZH2 siRNAs or EZH2 inhibitors. d, e Quantification of colony numbers of sorted DiD^low AM cells treated with EZH2 siRNAs or EZH2 inhibitors, **P < 0.01 by one-way ANOVA. f Western blot images showing EZH2 expression of DiD^low AM cells treated with EZH2 siRNAs. g Representative images showing spheres of sorted DiD^low AM cells treated with two EZH2 inhibitors; scale bar, 100 μm. h, i Quantification of sphere number and diameter in sorted DiD^low AM cells treated with or without EZH2 inhibitors, ***P < 0.001 by one-way ANOVA. j Representative images showing spheres of sorted DiD^low AM cells treated with two different EZH2 siRNAs; scale bar, 100 μm. (k, l) Quantification of sphere number and diameter in sorted DiD^low AM cells transfected with siRNA control or EZH2 siRNAs, ***P < 0.001 by one-way ANOVA