Fig. 1
From: Periodontitis exacerbates pulmonary hypertension by promoting IFNγ+ T cell infiltration in mice
Periodontitis (PD) aggravates pulmonary hypertension (PH) in mice. a Schematic illustration of the experimental design. PD was induced by ligation of molar (at week -2) in combination with 7 times of inoculation of subgingival plaques (PL) taken from patients with periodontitis. Mice were exposed to normoxia (21% O2) or hypoxia (10% O2) for 3 weeks in a ventilated chamber. b, c Right ventricular systolic pressure (RVSP) b and Fulton index c determined by the ratio of right ventricle (RV) weight to left ventricle (LV) weight plus septum weight (RV/(LV + S)). n = 8:8:8:12 for RVSP, n = 5:5:16:13 for Fulton index. d, e Representative echocardiography images d and measurements of pulmonary artery (PA) function by PA acceleration time to ejection time (PAT/PET) ratio e after exposure to normoxia or hypoxia for 3 weeks. n = 5:5:11:9. f Representative wheat germ agglutinin (WGA) staining of RV sections. g Quantification of RV cardiomyocyte (RVCM) area based on WGA staining. n = 8:8:9:9. h Representative hematoxylin and eosin (H&E) staining of lung sections. i Quantification of medial thickness as a percentage of external diameter of PAs. n = 4:4:9:9. j Representative immunofluorescence staining of alpha-smooth muscle actin (α-SMA) in lung sections. k Quantification of α-SMA+ area as a percentage of cross-sectional area (CSA) of PAs. n = 4:5:5:5. Ctrl, nonligatured control. PD, ligature-induced periodontitis with oral infection of PL. PH, pulmonary hypertension. Data are presented as mean ± SEM. Two-way ANOVA b, c, e, g, i, k was used for statistical analysis. ns, not significant. *P < 0.05. **P < 0.01. ***P < 0.001. ****P < 0.000 1
