Fig. 5

Surgical excision of cLNs alleviates the exacerbating effects of P. zoogleoformans on PH. a Schematic illustration of the experimental design. b, c RVSP b and c Fulton index determined by the ratio of (RV/(LV + S)). n = 5: 5:7:7 for RVSP, n = 7: 4:5:5 for Fulton index. d, e Representative echocardiography images d and measurements of PA function by PAT/PET ratio e after exposure to hypoxia for 3 weeks. n = 5: 5:6:5. f Representative H&E staining and immunofluorescence staining of α-SMA of lung sections. g Quantification of medial thickness as a percentage of external diameter of PAs. n = 5: 5:5:5. h Quantification of α-SMA⁺ area as a percentage of cross-sectional area (CSA) of PAs. n = 5: 5:5:5. i Representative flow cytometry plots of CD4⁺IFNγ⁺ T cells in lungs. j, k Quantification of the percentage of CD45⁺ cells and number of CD4⁺IFNγ⁺ T cells in lungs. n = 8:7. CE, cLN excision. Data are presented as mean ± SEM. Two-way ANOVA b, c, e, g, h and Student’s t test j, k were used for statistical analysis. ns not significant. *P < 0.05. **P < 0.01. ***P < 0.001