Fig. 6

IFNγ neutralization constrains P. zoogleoformans-induced exacerbation of PH. a Schematic illustration of the experimental design. b, c RVSP b and c Fulton index determined by the ratio of (RV/(LV + S)). n = 5: 5:7:7 for RVSP, n = 5: 5:6:6 for Fulton index. d, e Representative echocardiography images d and measurements of PA function by PAT/PET ratio e after exposure to hypoxia for 3 weeks. n = 5: 5:10:7. f Representative WGA staining of RV sections. g Quantification of RVCM area based on WGA staining. n = 5:5:5:5. h Representative H&E staining and immunofluorescence staining of α-SMA of lung sections. i Quantification of medial thickness as a percentage of external diameter of PAs. n = 5:5:5:5. j Quantification of α-SMA⁺ area as a percentage of cross-sectional area (CSA) of PAs. n = 5:5:5:5. Anti-IFNγ, IFNγ-neutralizing antibodies treatment. Data are presented as mean ± SEM. Two-way ANOVA b, c, e, g, i, j was used for statistical analysis. *P < 0.05. **P < 0.01. ***P < 0.001