Fig. 1 | International Journal of Oral Science

Fig. 1

From: Exosomal miR-17-5p derived from epithelial cells is involved in aberrant epithelium-fibroblast crosstalk and induces the development of oral submucosal fibrosis

Fig. 1

Epithelial cell-derived exosomes are transported to the primary fibroblasts, which promotes the myofibroblast differentiation phenotype. PBS-Exo (exosomes derived from epithelial cells treated with PBS), Arecoline-Exo (exosomes derived from epithelial cells treated with Arecoline), Arecoline+GW4869-Exo (exosomes derived from epithelial cells treated with Arecoline and GW4869). a Fibroblasts was incubated with DiI-labeled (red) exosomes from epithelial cells (50 μg) for 30 min and fixed for fluorescence staining. Nuclei were stained with DAPI (blue). Scale bars, 100 μm. b Immunostaining for fibrotic markers collagen type I and α-SMA in primary fibroblast with PBS-, Arecoline- and Arecoline+GW4869-Exo was evaluated by microscopy. DAPI (blue) was used for nuclear staining. Scale bar, 100 μm. c Immunoblotting of collagen type I and α-SMA by PBS-, Arecoline- and Arecoline+GW4869-Exo in primary fibroblasts. Sirius Red total collagen assay (d), collagen contraction assay (e, f) and transwell migration assay (g, h) were determined for fibroblast. n = 3–5 technical replicates, representative of two or three assays. Scale bars, 100 μm. Statistics: mean ± SEM, unpaired, one-way ANOVA (d, f, h), ***P < 0.001, ****P < 0.000 1

Back to article page