Fig. 6 | International Journal of Oral Science

Fig. 6

From: Exosomal miR-17-5p derived from epithelial cells is involved in aberrant epithelium-fibroblast crosstalk and induces the development of oral submucosal fibrosis

Fig. 6

miR-17-5p inhibits the expression of E3 ubiquitin-protein ligase WWP1. a After transfected with miR-17-5p or anti-miR-17-5p for 48 h, the expression of miR-17-5p in fibroblasts was detected by qRT-PCR. Immunoblotting (b) and qRT-PCR (c) detection of TGFBR1 expression in fibroblasts transfected by miR-17-5p and anti-miR-17-5p. d UbiBrowser 2.0 online site predicts E3 ubiquitin ligase with TGFBR1 as substrate. e The annotation list for the predicted E3 ligases of TGFBR1. f qRT-PCR detection of E3 ubiquitin ligase (NEDD4L, SMURF1, SMURF2, TRAF6, VHL, WWP1) expression level in fibroblast transfected by miR-17-5p and anti-miR-17-5p. Statistics: mean ± SEM, one-way ANOVA, n.s.: no significance, ****P < 0.000 1

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