Fig. 7 | International Journal of Oral Science

Fig. 7

From: Exosomal miR-17-5p derived from epithelial cells is involved in aberrant epithelium-fibroblast crosstalk and induces the development of oral submucosal fibrosis

Fig. 7

miR-17-5p inhibits WWP1-mediated degradation of TGFBR1 protein in fibroblasts. a Fibroblast transfected with miR-17-5p then incubated with cycloheximide (CHX, 100 μg/mL) for indicated time points. The protein levels of WWP1 and TGFBR1 were determined at indicated time points by Immunoblotting. b Fibroblasts transfected with miR-17-5p and anti-miR-17-5p then incubated with MG-132 (10 μM, 6 h). The protein levels of TGFBR1 were determined by Immunoblotting. c The expression localization of WWP1 (green) and TGFBR1 (red) in fibroblast was detected by Immunostaining. Scale bar: 50 mm. d, e Co-IP and Immunoblotting detected the interaction between WWP1 and TGFBR1 in fibroblast. f Co-IP and Immunoblotting detected the ubiquitination of TGFBR1 mediated by WWP1 and miR-17-5p overexpressed. g, h The expression of WWP1 and TGFBR1 protein levels in miR-17-5p overexpressed or inhibited in fibroblast was detected by Immunoblotting

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