Fig. 9 | International Journal of Oral Science

Fig. 9

From: Exosomal miR-17-5p derived from epithelial cells is involved in aberrant epithelium-fibroblast crosstalk and induces the development of oral submucosal fibrosis

Fig. 9

miR-17-5p induces collagen accumulation and promotes the progression of OSF by TGF-β in vivo. a Schematic overview of mouse experimental design. Mice were injected with 30 μL PBS or Bleomycin into the bilaterally buccal mucosa every other day for six weeks. Then, mice were simultaneously treated with miR-NC (Ctrl), miR-17-5p agomir and SB525334 for another two weeks. b HE, Masson and Sirius red staining of mice oral mucosa tissues. Scale bar, 200 μm. Quantification of Masson staining for collagen fraction. Scale bar, 200 μm. d Hydroxyproline content of mice oral mucosa tissues. c Quantification of Masson staining for skin tissues collagen fraction, n = 6 for each group. e Immunoblotting of fibrotic markers (Fibronectin, Collagen type I and α-SMA), Smad7, TGFBR1 and p-Smad2 pathway. f The levels of miR-17-5p of mice blood-derived exosomes. Statistics: mean ± SEM, n = 6 for each group, one-way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.000 1

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