Fig. 7 | International Journal of Oral Science

Fig. 7

From: Integrative single-cell and bulk transcriptomes analyses reveals heterogeneity of serine-glycine-one-carbon metabolism with distinct prognoses and therapeutic vulnerabilities in HNSCC

Fig. 7

IMPDH inhibitor represses HNSCC cell progression through provoking GTP-exhaustion nucleolar stress. a Sketch map of de novo purine synthesis pathway. b GO enrichment analysis was performed on the differentially expressed genes between low-SGOC and high-SGOC malignant cells in scRNA-seq cohort. c LC was performed to measure the GTP and ATP contents in CAL27 cells treated with DMSO or MPA. d Effects of MPA treatment on nuclear size and morphology of HNSCC cells. e The protein levels of nucleolar stress markers were measured by western blot. GAPDH was used as the loading control. After treating with DMSO, MPA (10 μmol/L), or MPA and guanosine (100 μmol/L), and cell viability was analysed by CCK-8 assay (f), the localizations of GNL3 and NPM1 were determined using immunofluorescence imaging (g). Mean ± s.d.; *P < 0.05; (c, d) Student’s t tests, (f) one-way ANOVA. Scale bars, (d) 40 μm, (g) 20 μm

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