Fig. 3

BMAL1 functions in the regulation of GSDMD-mediated pyroptosis. a, f The representative images of cell morphology in indicated groups examined by SEM (scale bar: Low magnification = 50 μm; High magnification = 10 μm) b, g Quantification of LDH activity in mGFs treated with P. gingivalis LPS and Bmal1 siRNA (n = 5) or overexpression plasmid (n = 4). c, h The representative images of PI staining in indicated groups were examined by LCFM (scale bar: 100 μm). d, i Relative mRNA expressions of genes related to GSDMD-mediated pyroptosis in indicated groups measured by qRT-PCR (n = 3). e, j Relative levels of proteins related to NLRP3 inflammasome signaling in indicated groups detected by western blot (n = 3). Data are represented as the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001. Abbreviations: LPS lipopolysaccharide, LDH lactate dehydrogenase, PI propidium iodide, SEM scanning electron microscope, LCFM laser confocal fluorescence microscopy