Fig. 1

Extraction and identification of extracellular vesicles. a Schematic diagram of the extracellular-vesicle extraction process. b Transmission electron micrographs of mandible-EVs. Scale bar: 100 nm. c Particle sizes measured by nanoparticle tracking analysis. d Western blotting characterization of EV markers. e Topography of mandible-EVs detected by AFM. f Topography of mandible-EVs detected by SEM. g Raman spectra of mandible-EVs. h Representative 600 MHz 1H NMR of mandible-EVs. Sample, conditioned media collected after culture of miniature pig mandible with tooth germ tissue removed