Fig. 2

NUP62 regulates human dental pulp stem cell (HDPSC) senescence. a, b Western blotting for NUP62, P21, and GAPDH (loading control) was performed using lysates from HDPSCs transfected with Lentiviral vector and overexpressing NUP62. c, d Immunofluorescence staining of γH2AX and quantification of fluorescence intensity (n = 5). Scale bar, 50 μm. e, f Representative images of senescence-associated β-galactosidase (SA β-gal) staining of O-HDPSCs and quantification of positive cells (n = 10). Scale bar, 50 μm. g, h Western blot analyses of NUP62, P53, and GAPDH expression in HDPSCs transfected with empty lentiviral control or in which NUP62 expression was via lentiviral delivery of shRNAs targeting 2 different sequences (#1, #2). i, j Representative images of SA β-gal staining and quantification of positive cells (n = 10). Scale bar, 50 μm. k, l Immunofluorescence staining of γH2AX and quantification of fluorescence intensity (n = 5). Scale bar, 50 μm. *P < 0.05, **P < 0.01 and ***P < 0.001