Fig. 4

Overexpression of NUP62 ameliorates senescence-associated hallmarks of O-human dental pulp stem cells (HDPSCs) via NSD2 upregulation. a Heatmap of differentially expressed genes in HDPSCs with and without NUP62 overexpression (n = 9). b Volcano plot indicating the differential expression of genes in HDPSCs with and without NUP62 overexpression. c Quantitative RT‒PCR results of representative genes (n = 3). d Heatmap of representative epigenetics-related genes in HDPSCs with and without NUP62 overexpression (n = 9). e, f Western blot analyses of NUP62, P21, NSD2, and GAPDH expression in HDPSCs transfected with lentiviral vector or overexpressing NUP62. g Quantitative RT‒PCR results for NSD2 (n = 3). h, i Western blot analyses of NUP62, P21, NSD2 and GAPDH expression in HDPSCs transfected with a lentiviral vector or NUP62 overexpression lentivirus and control siRNA (NC) or NSD2 siRNA. j Representative images of SA β-gal staining and quantification of positive cells (n = 10). Scale bar, 50 μm. *P < 0.05, **P < 0.01 and ***P < 0.001