Fig. 5

NUP62 mediates epigenetic reprogramming by upregulating the global levels of H3K36me2 and H3K36me3. a, b Western blot analyses of H3K36me2, H3K36me3, H3K27ac, H3K4me3, and H3K9me3 in human dental pulp stem cells (HDPSCs) transfected with lentiviral vectors or overexpressing NUP62. c, d Immunofluorescence staining and quantification of H3K36me2, respectively (n = 5). Scale bar, 50 μm. e, f Immunofluorescence staining and quantification of H3K36me3, respectively (n = 5). Scale bar, 50 μm. g Heatmap of global H3K36me2 and H3K36me3 CUT&Tag-seq signals from HDPSCs transfected with empty lentiviral vector or NUP62 overexpression lentivirus (n = 2). h Gene track view of normalized bigWig reads at the promoters of SIRT6, HMGA1 and HMGA2 in HDPSCs transfected with a lentiviral vector or overexpressing (n = 2). *P < 0.05, **P < 0.01 and ***P < 0.001