Fig. 4 | International Journal of Oral Science

Fig. 4

From: Expansion of functional human salivary acinar cell spheroids with reversible thermo-ionically crosslinked 3D hydrogels

Fig. 4

3D NS-SV-AC spheroid formation in hydrogels. Cells were seeded into a AG, b AGC, or c AGHA hydrogels and cultured for 14 days. Live/dead assay confocal imaging was performed to determine spheroid morphology (a–c), viability (d), and size (f). Metabolic activity was measured using WST-1 assay (e). Green, represents live cells; red, dead cells; blue, nuclear staining. Magnification/scale bar: ×20/20 μm. Data presented as mean ± SD, n ≥ 3, N.S., no significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.000 1. Viability and metabolic activity data are presented as fold of change and normalized to live cells at day 0

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