Fig. 5 | International Journal of Oral Science

Fig. 5

From: Expansion of functional human salivary acinar cell spheroids with reversible thermo-ionically crosslinked 3D hydrogels

Fig. 5

Analysis of key acinar cell markers in 3D NS-SV-AC spheroids. mRNA transcript levels were analyzed by RT-qPCR for acinar-specific markers expressed in 2D (plastic, 4 days in culture) and 3D platform (AGHA, 14 days in culture) (a–d). Data were normalized to GAPDH values. Data presented as mean ± SD, n ≥ 3, ***P < 0.001, ****P < 0.000 1. Immunodetection of acinar markers in NS-SV-AC cells growing in 2D (on plastic) and 3D platforms (in AGHA gels). 2D maximum projection of markers expressed by cells in 2D (e–h) or 3D (i–l) systems. For 2D culture images (e–h), the bright field was included in the merged images to increase the contrast of individual cells. Green, AQP5 or NKCC-1; red, ZO-1 or α-amylase; blue, nuclear staining. Magnification ×20, scale bar 20 µm

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