Fig. 6 | International Journal of Oral Science

Fig. 6

From: Expansion of functional human salivary acinar cell spheroids with reversible thermo-ionically crosslinked 3D hydrogels

Fig. 6

Functional analysis of NS-SV-AC spheroids cultured for 14 days in AGHA hydrogels. Cells and spheroids were stimulated with isoprenaline, and the amylase activity was measured by colorimetric assay. The amylase activity was reported as nmol/mL per min (milliunits), where one unit is the amount of amylase that cleaves ethylidene-pNP-G7 to generate 1.0 μmole of p-nitrophenol per minute at 25 °C. Data presented as mean ± SD, N.S. not significant, n ≥ 3, *P < 0.05. 2D maximum projection (b, c) and 3D reconstruction (d, e) of immunodetected α-amylase in non-stimulated (b, d) and stimulated (c, e). The panel at the very right is a close up of the selected area (white dotted box), and arrows indicate the α-amylase granules: red, α-amylase; green, F-actin; blue, nuclear staining. Magnification ×20, scale bar 20 µm

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