Table 1 Summary of hydrogel performance as a 3D cell expansion platform

From: Expansion of functional human salivary acinar cell spheroids with reversible thermo-ionically crosslinked 3D hydrogels

Items

Hydrogel composition

AG

AGC

AGHA

Viability/%

 Day 3

84.20 ± 3.74

90.87 ± 0.76

90.57 ± 0.65

 Day 7

85.75 ± 2.89

84.20 ± 1.75

89.71 ± 1.71

 Day 10

84.26 ± 2.42

87.78 ± 0.41

90.41 ± 1.24

 Day 14

87.82 ± 2.56

81.00 ± 1.46

93.21 ± 1.96

Maximum spheroid surface area/μm2

 Day 3

1 742.70

1 996.08

1 582.03

 Day 7

4 449.46

4 461.82

4 653.40

 Day 10

7 601.17

10 351.19

12 050.64

 Day 14

28 056.36

41 225.55

61 204.89

Dead cells in spheroid core (day 14)

 

Few cells

Yes

No

Performancea

 

3

3

5

  1. The viability percentage was determined by culture per day per condition and not normalized with day 0 values. Data presented as mean ± SD, n ≥ 3
  2. ND not determined
  3. aHydrogel and culture performance as cell expansion system: arbitrary scale considering viability, metabolic activity (proliferation), spheroid formation, spheroid size, and presence of dead cells in their core. 1: very poor, 2: poor, 3: average, 4: good, 5: excellent
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