Fig. 5 | International Journal of Oral Science

Fig. 5

From: Osteomodulin modulates the inflammatory responses via the interleukin-1 receptor 1/nuclear factor-κB signaling pathway in dental pulpitis

Fig. 5

IL1R1/NF-κB signaling pathway might be involved in the OMD-modulated inflammation process. a KEGG enrichment analysis revealing the significant enrichment of NF-κB and TNF signaling pathways in OMD-overexpressing hDPSCs. b Cluster expression heat map showing the changes in key genes related to NF-κB and TNF signaling pathways. c Cluster heatmap showing the expression of co-occurring differentially expressed genes related to immune response. d RT-qPCR showing the expression alteration of IL1R1 in hDPSCs after the application of rhOMD (0.5 and 1 µg/mL) for 24 h. e RT-qPCR results demonstrating the inhibitory effect of OMD on the IL1R1 expression in NC and siOMD hDPSCs. f WB showing the protein level of IL1R1 and the ratio of p-p65/p65 in hDPSCs induced by 1 μg/mL of LPS alone or combined 1 μg/mL of LPS and 1 μg/mL of rhOMD for 0, 6, 12, or 24 h. g and h Quantitative analysis of the protein expression in (f). The results are expressed as mean ± SD (ns means no significance; *P < 0.05, **P < 0.01, and ***P < 0.001)

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