Fig. 6
Blockage of IL1R1 attenuated the increased inflammation generated by OMD deficiency. a IP assays were performed in HEK293T cells transfected with HA-OMD and Flag-IL1R1. IB analysis with indicated antibodies was shown. b Three-dimensional illustration of the interaction between OMD (the green chain) and IL1R1 (the blue chain), with the yellow dotted lines representing the hydrogen bond interaction. c Two-dimensional illustration displaying detailed amino acids involved in the interaction. The amino acids in the OMD protein chain are marked in green, whereas those in the IL1R1 chain are marked in blue. d mRNA expression of TNFα and IL-1β in siOMD hDPSCs induced by 1 μg/mL of LPS alone, 1 μg/mL of LPS and 1 μg/mL of rhOMD combined, or 1 μg/mL of LPS and 1 μg/mL of Raleukin combined. e–g Immunofluorescence staining and quantitative results showing TNFα and IL-1β expression in the pulp tissues of OC-Cre; Omdfl/fl mice treated with 1 mg/kg of LPS alone, 1 mg/kg of LPS and 1 mg/kg of rmOMD combined, or 1 mg/kg of LPS and 1 mg/kg of Raleukin combined. Representative images in (f) and (g) were obtained under ×5 magnification (scale bar: 500 μm) and ×20 magnification (scale bar: 100 μm). Results are expressed as mean ± SD (ns, no significance; *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.000 1)
