Fig. 4

AP waveform-dependent molecular profiles in Piezo2-ZsGreen⁺ DPA neurons. a Representative gel images showing scRT-PCR products from no hump-AP and hump-AP in Piezo2-ZsGreen⁺ DPA neurons. Predicted sizes for selected markers: Th (Tyrosine hydroxylase): 152 bp, Trpv1 (TRPV1): 115 bp, Scn1a (Na_V1.6): 123 bp, Scn8a (Na_V1.6): 97 bp, Scn9a (Na_V1.7): 144 bp, Scn10a (Na_V1.8): 190 bp, Scn11a (Na_V1.9): 209 bp, and Gapdh: 282 bp. Negative control (NC) refers to a sample of bath solution. Gene names are indicated by their gene symbols. n = 6 neurons in the no hump-AP and n = 16 neurons in the hump-AP from 8 mice (scRT-PCR was performed on samples that underwent patch-clamp recordings in Fig. 3a–i). b Radar plots summarizing the proportion of each gene’s expression in no hump-AP and hump-AP Piezo2-ZsGreen⁺ DPA neurons, respectively. c Summary of scRT-PCR results of Na_V1.8 and Na_V1.9 in no hump-AP or hump-AP Piezo2-ZsGreen⁺ DPA neurons