Fig. 5

Co-expression and correlation patterns of VGSC isoforms and TRPV1 in Piezo2-ZsGreen⁺ DPA neurons. a–c Representative z-stacked confocal images showing Scn1a, Scn10a, and Scn11a mRNAs (red) in FG-labeled DPA neurons (blue) expressing Piezo2 (green). White arrowheads mark double-positive DPA neurons. Scale bars: 20 μm. d Proportion of Piezo2⁺ DPA neurons that express each marker gene. n = 2 mice for all groups. One-way ANOVA followed by Bonferroni’s multiple comparisons test: *P = 0.016 4, **P = 0.008 7. e Violin plots representing the cell body size distribution (µm²) of marker⁺ neurons (Scn1a: n = 25 neurons; Scn10a: n = 50 neurons; Scn11a: n = 27 neurons). Kruskal-Wallis followed by Dunn’s multiple comparisons test, *P = 0.029 3. f Representative z-stacked confocal images showing Trpv1 mRNAs (red) in Scn10⁺ or Scn11a⁺ (white) FG-labeled DPA neurons (blue) expressing Piezo2 (green). White arrowheads mark triple-positive DPA neurons. Scale bars: 20 μm. g Comparative analysis of the proportion of Trpv1⁺ DPA neurons among Piezo2⁺ DPA neurons and each marker⁺ DPA neuron group. n = 3 mice for both groups. Unpaired Student’s t test: *P = 0.010 2. Data are shown as mean ± SEM. h–j Scatter plots displaying individual data points (black) from FG-labeled DPA neurons, with Pearson correlation coefficients (R), P-values, and fitted linear regression lines (blue) indicating the correlation trends between Piezo2 and Scn10a/Scn11a/Trpv1