Fig. 7

Residual type III cells seem to give rise to clones in the suprabasal layer during the regenerative phase. a Confocal images of CVP sections from T1r2-tdTomato mice at 4 weeks post-GLx, immunostained with anti-Krt14 (green) and anti-tdTomato (red). DAPI (blue) was used for nuclei counterstaining. b–d Confocal images of CVP from non-operated control Pirt-tdTomato mice and Pirt-tdTomato mice at 3 (c) or 4 weeks post-GLx (b, d) (n = 3, 3), immunostained with anti-Krt14 (green) and anti-tdTomato (red). DAPI (blue) was used for nuclei counterstaining in (a–d). e Confocal images of CVP from non-operated control and at 4 weeks post-GLx Pirt-tdTomato mice (n = 3, 3), immunostained with anti-Krt8 (green) and anti-tdTomato (red) with DAPI (blue) for nuclei staining. f Confocal images of CVP sections from B6N mice at 2 weeks post-GLx, immunostained with anti-Krt14 (green) and anti-Car4 (red). Arrowhead indicates tdTomato-positive cells in the suprabasal layers. Arrow indicates Krt14 expression in tdTomato-expressing cells. Asterisk denotes colocalization of anti-Car4 and anti-Krt14 signal. Scale bars, 50 μm