Fig. 4 | International Journal of Oral Science

Fig. 4

From: LncRNA EUDAL shapes tumor cell response to hypoxia-induced constitutive EGFR activation and promotes chemoresistance in oral cancer

Fig. 4

Binding of EUDAL to EGFR prevents its ubiquitination and endocytosis. a Left: The ectopic expression of EUDAL wt blocked the interaction between c-Cbl/Grb2 and EGFR, and attenuated subsequent ubiquitination in hypoxic HN4 cells. Right: Silencing of EUDAL promoted binding of c-Cbl/Grb2 to EGFR and elevated EGFR ubiquitination levels in hypoxic CAL-27 cells (n = 3). b pEGFR endocytosis rate in hypoxic HN4 and CAL-27 following indicated treatments. c Flow cytometric analysis of membrane-localized EGFR on hypoxic HN4 and CAL-27 cells (n = 3). Data are represented as mean ± SD. ns no significance; ****Endocytosis rate % < 0.000 1; one-way ANOVA with Turkey’s Honestly Significant Difference test (c). Baf Bafilomycin A1, wt wild-type, EUDAL Del-mut a lncRNA mutant with an EGFR-binding motif deletion (Δ254–305 nt); KD knockdown

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