Fig. 5

Binding of EUDAL to EGFR prevents its endocytosis and lysosomal degradation. a The colocalization of pEGFR with the lysosome marker LAMP1 in hypoxic HN4 and CAL-27 cells following indicated treatments, as shown by confocal microscopy images. The colocalization of pEGFR (red) and LAMP1 (green) was estimated with plot profile using ImageJ. Correlation coefficient R > 0.6 was considered well-collocated, while R < 0.6 was taken as poorly collocated. Bar, 20 µm. Percentage of cells with high EGFR intensity was indicated. b Representative immunoblot images of pEGFR and total EGFR levels after CHX treatment following the indicated treatments. Data were from representative results of at least three independent experiments. Pearson’s R (a). Baf Bafilomycin A1, wt wild-type, EUDAL Del-mut a lncRNA mutant with an EGFR-binding motif deletion (Δ254–305 nt), KD knockdown