Fig. 4
The neurogenic activity of bioceramics-containing composite hydrogels. a Proliferation of SCs cultured with composite hydrogels for 1, 3, and 5 days (n = 5). b Morphology of SCs on the surface of these hydrogels. c Crystalline violet staining images of the migrated SCs after cultured with composite hydrogels. d Quantitative statistics results of migrated SCs (n = 6). e Expression of neurotrophic factor and myelination-related genes in SCs cultured with composite hydrogels for 5 days (n = 3). Representative immunofluorescent images of f S100 and g GDNF proteins in SCs after cultured with GelMA and GelMA-5LCS hydrogel for 5 days. (Blue: cell nuclei; Green: cytoskeleton; Red: proteins). *P < 0.05, **P < 0.01, ***P < 0.001. GelMA-5LCS composite hydrogels could effectively enhance the proliferation, migration, and neurogenic differentiation activity of SCs
