Fig. 7
Bioceramics-containing composite hydrogels mediated the neurogenesis- odontogenesis couples. a Schematic diagram of the effects of SCs-derived condition medium on the migration and odontogenic differentiation of DPSCs. Conditioned medium was collected from SCs treated with different hydrogels for 2 days, and denoted as Control-CM, GelMA-CM, and GelMA-5LCS-CM, respectively. b Representative crystalline violet staining images of DPSCs after cultured with different conditioned medium. c Quantitative statistical analysis of the migrated DPSCs (n = 6). Representative immunofluorescent staining images of d DMP-1, e DSPP, and f OPN in DPSCs after cultured with different conditioned medium for 5 days (Blue: cell nuclei; Green: cytoskeleton; Red: proteins). Semi-quantitative analysis of g DMP-1, h DSPP and i OPN proteins expression of DPSCs (n = 5). *P < 0.05, **P < 0.01, ***P < 0.001. Bioceramics-containing composite hydrogels mediated the neurogenesis-odontogenesis couplings
